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Công bố quốc tế: Độc tính và hoạt tính chống tăng sinh của dịch chiết ethanol từ cây Phòng phong thảo (Anisomeles indica) trên tế bào ung thư cổ tử cung Hela và trên phôi cá ngựa vằn

Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol

Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos

Nguyen T. Bich-Loan 1,2, Kieu Trung Kien 1, Nguyen Lai Thanh 1, Nguyen T. Kim-Thanh 1, Nguyen Quang Huy 1,Pham The-Hai, Marc Muller 3, Amandine Nachtergael 2, Pierre Duez 2 and Nguyen Dinh Thang 1,*

1 Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi 100000, Vietnam; (N.T.B.-L.); (K.T.K.); (N.L.T.); (N.T.K.-T.); (N.Q.H.); (P.T.-H.)
2 Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), 7000 Mons, Belgium; (A.N.); (P.D.)
3 Laboratory for Organogenesis and Regeneration, GIGA-R, Department Life Sciences, University of Liege, 4000 Liege, Belgium;
* Correspondence:

Abstract: In this study, we showed that crude extract of Anisomeles indica (AI-EtE) expressed its  toxicity to HeLa cells with an IC50 dose of 38.8 µg/mL and to zebrafish embryos with malformations, lethality and hatching inhibition at 72-hpf at doses higher than 75 µg/mL. More interestingly, flow cytometry revealed that AI-EtE significantly promoted the number of cells entering apoptotic. Accordingly, the transcript levels of BAX, CASPASE-8, and CASPASE-3 in the cells treated with AI-EtE at IC50 dose were 1.55-, 1.62-, and 2.45-fold higher than those in the control cells, respectively. Moreover, treatment with AI-EtE caused cell cycle arrest at the G1 phase in a p53-independent manner. Particularly, percentages of AI-EtE-treated cells in G1, S, G2/M were, respectively 85%, 6.7% and 6.4%; while percentages of control cells in G1, S, G2/M were 64%, 15% and 19%, respectively. Consistent with cell cycle arrest, the expressions of CDKN1A and CDNK2A in AI-EtE-treated cells were up-regulated 1.9- and 1.64-fold, respectively. Significantly, treatment with AI-EtE also decreased anchorage-independent growth of HeLa cells. In conclusion, we suggest that Anisomeles indica can be considered as a medicinal plant with a possible use against cervical cancer cells; however, the used dose should be carefully monitored, especially when applying to pregnant women.
Keywords: medicinal plant; Anisomeles indica; HeLa cell; zebrafish; apoptosis; cell cycle arrest; gene expression; colony formation

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